Multiplex Core

Technology Background

Bead-based multiplex arrays are commonly used for the detection of various biomarkers and proteins. This technique uses microsphere beads, coated with monoclonal antibodies against specific proteins, to simultaneously measure multiple analyte concentrations in various sample types.

  • body fluids (serum, saliva, plasma, GCF, wound fluid, etc)
  • cell extracts
  • culture supernatants
  • Target species including human, mouse, rat, non-human primate, canine, porcine, etc.

Luminex® and their partners have developed many applications using the XMAP® Technology platform, including the following fields of life science research:

  • Cancer Markers
  • Cardiovascular
  • Cell Signaling
  • Cellular Metabolism
  • Immunology
  • Gene Expression Profiling
  • Genotyping
  • Endocrinology
  • Isotyping
  • Matrix Metalloproteinases
  • Metabolic Endocrinology
  • Neurobiology
  • Transcription Factors
  • Toxicity

Multiplex Core

The Forsyth Multiplex Core, established in 2013, offers three main platforms:

  • Luminex® 200™
  • FLEXMAP 3D™
  • AYOXXA Lunaris Technology

All platforms operate 96-well plate based assays. FLEXMAP 3D™ and AYOXXA also offer 384-well plate capacity.

Plate capacity: up to 80 samples in singlet with up to 16 wells designated as standards for antigen-antibody based assays (for 96-well format).

Capabilities

  • Multiplex protein detection using antibody-based assays of up to 100 analytes or 500 analytes in a single microplate well using the Luminex® 200™ or FLEXMAP 3D™, respectively.
  • Ability to custom coat beads with various mammalian and non-mammalian molecules such as nucleic acids, receptor-ligands, and siRNA. These require custom-plexing, assay development/optimization, and additional pricing.
  • Both Luminex systems use BioPlex Manager, from Bio-Rad for acquisition and computation of data.
    • Allows easy transfer of the data to excel or other user-friendly programs.
    • In addition to the raw and computed data on concentrations and fluorescence intensity, standard curves, %CV, internal control samples, plate-to-plate variation, and standard deviation are only a few of the data output measures.

Contact: the Multiplex Core  by email to multiplex@forsyth.org with any inquires or to schedule a meeting.