Forsyth maintains specialty Core Services that provide specialized equipment and expertise for its investigators and, on a fee-for-service basis, to researchers from other institutions. Visit the specific web pages below and/or download additional details about technology, services and scheduling.
The Imaging Core allows for light microscopy and, for internal use only, scanning electron microscopy on a Zeiss Evo LS10 scanning electron microscope with Peltier cooling stage and Oxford INCA 250 microanalysis system for EDS. Light microscopes include a Zeiss LSM 880 Confocal MIcroscope upright microscope with a multialkali detector capable of capturing 3 channels simultaneously, an Airyscan detector, Fast Airyscan, 7 laser lines, environmental housing, temperature control, and 20x CLARITY objective for imaging cleared tissue specimens, Zeiss LSM 780 confocal microscope with GAsP 34 channel detector for spectral imaging Zeiss Axio Observer inverted microscope with epi-fluorescence illumination and Colibri LED equipped with a Hamamatsu Orca-Flash4 camera, a Zeiss Stemi SV11, Leica DMRE light microscope, Leica DMLS light microscope with Leica DC100 digital camera, and Leica DMR fluorescence microscope. Associated support services include sample preparation, ultrastructure, histology and image analysis of soft and mineralized tissue. Felicitas Bidlack, Director.
The Epidemiology and Biostatistics Core offers consultation services for study design and data management relating to biometry, epidemiology, and clinical trials. Jacqueline Starr, Director.
Flow cytometry is a method by which cellular or small particulate samples, suspended in a pressurized fluidic system, can be analyzed based on fluorescent properties or labels using an array of lasers, optics, and computerized sensors. This allows for detection of extracellular and intracellular markers including proteins, fluorescent stains, or nucleic acid dyes at a single-cell level. There is a large array of applications for slow cytometry, including: immunophenotyping, transduction/transfection efficiency, extracellular cytokine quantification, and cellular function assays. The Flow Cytometry Core provides consulting and support services for the detection of up to 14 different fluorescent parameters simultaneously, RBC contaminated samples, and high-throughput analysis using 96 or 384-well plates. The cell sorter allows for collecting cells/particulates of interest, after screening for positively selected markers, for down-stream applications, and is capable of sorting 4 separate cell populations simultaneously. Xiaozhe Han, Director.
The Histology Core provides consulting and support services for both soft tissue and precise hard tissue analysis. Services include: tissue processing, embedding, sectioning, and staining, as well as guidance on protocols. The Histology Core can embed samples in standard molds or custom molds in paraffin, resin or as frozen tissue for routine histology, special stains and immunohistological studies performed either by the investigator or Core Staff. Christine Kressirer, Director.
The Multiplex Core offers three platforms for bead-based multiplex arrays (AYOXXA Lunaris™ Technology, Luminex® 200™, FLEXMAP 3D™). Bead-based multiplex arrays are useful for the detection of various biomarkers and proteins. This technique uses microsphere beads, coated with monoclonal antibodies against specific proteins, to simultaneously measure multiple analyte concentrations in various sample types including: body fluids (serum, saliva, plasma, GCF, wound fluid, etc.), cell extracts and culture supernatants. Alpdogan Kantarci, Director. Contact the core at email@example.com. Learn more about the benefits.
The Mass Spectrometry Core at the Forsyth Center for Salivary Diagnostics provides mass spectrometry analysis and strategic consulting in proteomics for internal and external researchers. The Core houses state-of-the-art instrumentation (three mass spectrometers, a peptide synthesizer, a Biacore 3000 and associated instruments) and brings together expertise in proteomics, peptide synthesis and surface plasmon resonance. Markus Hardt, Director.
The Forsyth Micro Computed Tomography (μCT) Core offers micro 3D X-ray imaging of small samples in high resolution, and will provide images and quantitative analyses of internal structures of ex vivo samples without any destructive procedures. The Scanco μCT40 system allows for visualization, measurement, and quantification of the structure of mineralized specimens. Contrast reagents permit visualization of structures such as blood vessels. This system creates a three-dimensional model from a stack of two-dimensional X-ray images taken around a single axis of rotation. The high-resolution scanner can capture detail at a resolution of 6 to 32 microns. The non-destructive nature of this technology allows investigators to carry out complementary analyses (e.g. histology) of the same samples. Yang Hu, Manager.
The Forsyth Sequencing and Bioinformatics Core is currently undergoing reorgnization. For more information, please contact Alexis Kokaras, Sequencing Core Coordinator.