The Forsyth Institute maintains an Animal Facility to support the Institute’s research in oral and craniofacial biology, the human microbiome, oral immunology, and mineralized tissue biology. The facility occupies a total of 4200 sq ft, including animal holding rooms, procedure spaces, cage wash, IVIS imaging and a germ-free facility. All animals are maintained under SPF conditions, with a sentinel program in place for mouse housing. The facility has been fully accredited by AAALAC since 1977, is registered with USDA and maintains an assurance with PHS. The facility complies with all USDA, OLAW, and City of Cambridge requirements. 

The animal program is overseen by the Forsyth institutional animal care and use committee (IACUC), which performs protocol approval and program oversight for both internal and external projects. The IACUC meets six times per year. If interested in using the Forsyth Institute Animal Facility, please contact us at iacuc@forsyth.org to see if we can accommodate your needs.

IACUC Policies


The Bioinformatics Core is your resource for a variety of bioinformatics needs. Contact George Chen (tchen@forsyth.org), Director.


Forsyth is now offering Next Generation Sequencing (NGS) and comprehensive data analyses and interpretation for 16S rRNA gene amplicon sequences and other big data sequence applications through the new Forsyth Oral Microbiome Core (FOMC).

Analyses of previously obtained sequences are also offered. Many investigators are familiar with the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) as well as its predecessor the Human Oral Microbe Identification Microarray (HOMIM) for rapid 16S rDNA analyses of oral clinical samples. We continue in that tradition using state-of-the-art bioinformatic methodology, which will provide the best possible taxa identification.

Dr. Bruce Paster (bpaster@forsyth.org), an internationally known oral microbiome researcher, is the director of FOMC and Dr. George Chen (tchen@forsyth.org), director of Forsyth Bioinformatics Core, is a well-known bioinformatic specialist in oral microbiome research. The primary focus of the FOMC involves the microbial analysis of samples derived from the human oral cavity, however analyses of samples from all human, animal, and environmental sites are also available. Visit the FOMC website to learn more.


The Flow Cytometry Core offers consulting and support services for the detection of up to 14 different fluorescent parameters simultaneously, RBC contaminated samples, and high-throughput analysis using 96 or 384-well plates. The cell sorter allows for collecting cells/particulates of interest, after-screening for positively selected markers, downstream applications, and is capable of sorting four separate cell populations simultaneously. Contact Yang Hu (yhu@forsyth.org), Director.

Service TypeAcademic (per hour)Commercial (per hour)
Analysis$80$100
Cell Sorting$130$160
Consultation$60$70

The Histology Core offers consulting and support services for both soft tissue and precise hard tissue analysis. Services include: tissue processing, embedding, sectioning, staining, and guidance on protocols. The Histology Core can embed samples in standard molds or custom molds in paraffin, resin, or as frozen tissue for routine histology. The core can also provide special stains and immunohistological studies performed either by the investigator or core staff. Contact histology@forsyth.org. Pricing can be found here:


The Imaging Core maintains three light microscopes for internal and external use: 

·  The Zeiss Axio Observer inverted microscope with epi-fluorescence illumination by a Colibri.2 LED and equipped with a Hamamatsu Orca-Flash4 camera is ideal for live cell transmitted and reflected light imaging. 

·  The Zeiss LSM 780 confocal inverted microscope has 34 channel detectors for spectral imaging and linear unmixing capabilities for thick specimens and experiments with multiple fluorophores. Environmental control is also available for these two systems. 

·  The Zeiss LSM 880 confocal upright microscope has a multialkali detector capable of capturing three channels simultaneously, a 32-channel Airyscan detector for super-resolution, Fast Airyscan, environmental housing, and a 20x CLARITY objective for imaging cleared tissue specimens. 

Associated support services include sample preparation assistance, imaging, and image analysis guidance on a variety of sample types.

Contact: Jennifer Gundrum, Manager at jgundrum@forsyth.org) or Felicitas Bidlack, Director at fbidlack@forsyth.org.

External Rates:

MicroscopeAcademic (per hour)Commercial (per hour)
Zeiss Axio Observer Widefield$ 60$ 90
Zeiss LSM 780 Confocal$ 100$ 150
CO2 Incubation Unit$ 15$ 15
Zeiss LSM 880 Confocal$ 100$ 150
Training/assisted useAdditional $150Additional $150

The Mass Spectrometry Core at the Forsyth Center for Salivary Diagnostics offers mass spectrometry analysis and strategic consulting in proteomics for internal and external researchers. The Core houses state-of-the-art instrumentation—three mass spectrometers and associated instruments—and brings together expertise in proteomics, peptide synthesis, and surface plasmon resonance. Contact Markus Hardt, Director at mhardt@forsyth.org.


The Micro-Computed Tomography (μCT) Core offers micro 3D X-ray imaging of small samples in high resolution, and provides images and quantitative analyses of internal structures of ex vivo samples without any destructive procedures. The Scanco μCT40 system allows for visualization, measurement, and quantification of the structure of mineralized specimens. Contrast reagents permit visualization of structures such as blood vessels. This system creates a three-dimensional model from a stack of two-dimensional X-ray images taken around a single axis of rotation. The high-resolution scanner can capture detail at a resolution of 6 to 32 microns. The non-destructive nature of this technology allows investigators to carry out complementary analyses (e.g. histology) of the same samples. Contact: Yang Hu, Director at yhu@forsyth.org.

Service TypeAcademic (per hour)Commercial (per hour)
Assisted scanning$100$150
Consultation$150$150
Software analysis (Amira/Scanco)$20$30

The Multiplex Core offers three platforms for bead-based multiplex arrays (AYOXXA Lunaris™ Technology, Luminex® 200™, FLEXMAP 3D™). Bead-based multiplex arrays are useful for the detection of various biomarkers and proteins. This technique uses microsphere beads, coated with monoclonal antibodies against specific proteins, to simultaneously measure multiple analyte concentrations in various sample types, including: body fluids (serum, saliva, plasma, GCF, wound fluid, etc.), cell extracts, and culture supernatants. Contact: multiplex@forsyth.org.